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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZMYND10 Recombinant antibody (86430-1-RR)

    WB analysis using 86430-1-RR (same clone as 86430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86430-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZMYND10 Recombinant antibody (86430-1-RR)

    WB analysis using 86430-1-RR (same clone as 86430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86430-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86430-1-RR

    Affinity and Kinetic Characterization of 86430-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86430-1-RR against Human ZMYND10 were performed. The affinity constant is below 1 pM.

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ZMYND10 Recombinant antibody, PBS Only

ZMYND10 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 86430-1-PBS
Clone No.251276H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86430-1-PBS targets ZMYND10 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZMYND10 fusion protein Ag5808 相同性解析による交差性が予測される生物種
Full Name zinc finger, MYND-type containing 10
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033732
Gene Symbol ZMYND10
Gene ID (NCBI) 51364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75800
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).