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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZFYVE16 Polyclonal antibody

ZFYVE16 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13118

Synonyms

DKFZp686E13162, ENDOFIN, KIAA0305, ZFYVE16

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-13118

    IF Staining of HeLa using CL488-13118

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ZFYVE16 antibody (CL488-13118) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-13118 targets ZFYVE16 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE16 fusion protein Ag3811 相同性解析による交差性が予測される生物種
Full Name zinc finger, FYVE domain containing 16
Calculated molecular weight 1539 aa, 169 kDa
Observed molecular weight230-250 kDa
GenBank accession numberBC032227
Gene symbol ZFYVE16
Gene ID (NCBI) 9765
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZFYVE16 antibody CL488-13118Download protocol
Standard Protocols
Click here to view our Standard Protocols