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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of U-937 using 12306-1-AP (same clone as 12306-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of HeLa using 12306-1-AP (same clone as 12306-1-PBS)

    WB result of ZFP36L1/2 antibody (12306-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZFP36L1/2 transfected HeLa cells. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of RAW 264.7 using 12306-1-AP (same clone as 12306-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of RAW 264.7 using 12306-1-AP (same clone as 12306-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of HeLa using 12306-1-AP (same clone as 12306-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 3T3-L1 cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    WB analysis of 3T3-L1 using 12306-1-AP (same clone as 12306-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat ovary tissue using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat ovary tissue using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFP36L1/2 Polyclonal antibody (12306-1-AP)

    IF Staining of HepG2 using 12306-1-AP (same clone as 12306-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L1/2 antibody (12306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ZFP36L1/2 Polyclonal antibody, PBS Only

ZFP36L1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12306-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ZFP36L1, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12306-1-PBS targets ZFP36L1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFP36L1/2 fusion protein Ag2952 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 1
Calculated molecular weight 338 aa, 36 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC018340
Gene Symbol ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2737443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07352
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZFP36L1, also named as Butyrate response factor 1 or TPA-induced sequence 11b, is a 338 amino acid protein, which is phosphorylated by RPS6KA1 at Ser-334 upon phorbol 12-myristate 13-acetate (PMA) treatment. ZFP36 encodes tristetraprolin (TTP) the prototype of a small family of RBPs, called the ZFP36 family, that are characterised by highly conserved tandem CCCH zinc-finger RNA-binding domains [PMID: 10751406]. ZFP36 is a RBP that promotes RNA decay and negatively regulates the expression of the myogenic regulatory factor MyoD by binding to the 3'UTR of MyoD mRNA [PMID: 25815583]. Mouse satellite cells from Zfp36-deficient mice express increased amounts of MyoD and display impaired satellite activation, demonstrating a role for ZFP36 in the maintenance of quiescence [PMID: 25815583]. The functions of the ZFP36L1 and ZFP36L2 family members have not been evaluated in skeletal muscle stem cell fate, but have been shown to act redundantly to promote quiescence during lymphocyte development. ZFP36L1 has been implicated in the persistence of the marginal zone B lymphocyte population. ZFP36L1 exists three isoform through blasting on NCBI database and can be phosphorylated, so the range of the molecular weight of ZFP36L1 is about 40-50 kDa. (PMID: 26180518, PMID: 17030620). This antibody recognizes both ZFP36L1 and ZFP36L2.