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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of SKOV-3 using 66279-1-Ig (same clone as 66279-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

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ZEB1 Monoclonal antibody, PBS Only

ZEB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66279-1-PBS
Clone No.1H1F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66279-1-PBS targets ZEB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZEB1 fusion protein Ag17118 相同性解析による交差性が予測される生物種
Full Name zinc finger E-box binding homeobox 1
Calculated molecular weight 1125 aa, 124 kDa
Observed molecular weight 190-210 kDa
GenBank accession numberBC112392
Gene Symbol ZEB1
Gene ID (NCBI) 6935
RRIDAB_2881662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37275
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.