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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZDHHC5 Polyclonal antibody

ZDHHC5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21324

Synonyms

DHHC 5, DKFZp586K0524, KIAA1748, ZDHHC5, Zinc finger protein 375, ZNF375

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-21324

    FC experiment of HeLa using CL488-21324

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ZDHHC5 (CL488-21324) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21324-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-21324 targets ZDHHC5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZDHHC5 fusion protein Ag14891 相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 5
Calculated molecular weight 715 aa, 78 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC026967
Gene symbol ZDHHC5
Gene ID (NCBI) 25921
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ZDHHC5 antibody CL488-21324Download protocol
Standard Protocols
Click here to view our Standard Protocols