Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse kidney using 21324-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21324-1-AP
WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.
WB analysis of HEK-293 using 21324-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21324-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 21324-1-AP
IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.
IHC staining of human kidney using 21324-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21324-1-AP (ZDHHC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21324-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21324-1-AP (ZDHHC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 21324-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 21324-1-AP
Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HT-29 using 21324-1-AP
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 21324-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

ZDHHC5 Polyclonal antibody

ZDHHC5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21324-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5, Zinc finger DHHC domain-containing protein 5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HT-29 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 21 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 4 publications below
CoIP	See 2 publications below

Product Information

21324-1-AP targets ZDHHC5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14891 Product name: Recombinant human ZDHHC5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 313-662 aa of BC026967 Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV 相同性解析による交差性が予測される生物種
Full Name	zinc finger, DHHC-type containing 5
Calculated molecular weight	715 aa, 78 kDa
Observed molecular weight	77 kDa
GenBank accession number	BC026967
Gene Symbol	ZDHHC5
Gene ID (NCBI)	25921
RRID	AB_10732816
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9C0B5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Protocols

Product Specific Protocols
FC protocol for ZDHHC5 antibody 21324-1-AP	Download protocol
IF protocol for ZDHHC5 antibody 21324-1-AP	Download protocol
IHC protocol for ZDHHC5 antibody 21324-1-AP	Download protocol
IP protocol for ZDHHC5 antibody 21324-1-AP	Download protocol
WB protocol for ZDHHC5 antibody 21324-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D Authors - Gang Du View Article
mouse	WB	Adv Mater Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy Authors - Guoqing Zhu View Article
human	WB	Cancer Res ZDHHC2-mediated AGK palmitoylation activates AKT/mTOR signaling to reduce sunitinib sensitivity in renal cell carcinoma Authors - Yan Sun KO Validated View Article
human	WB	J Adv Res Neonatal sevoflurane exposures inhibits DHHC5-mediated palmitoylation of TfR1 in oligodendrocytes, leading to hypomyelination and neurological impairments Authors - Huiqing Liu View Article
mouse	WB	Mol Cell ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation Authors - Sihao Zheng View Article
mouse	WB	Sci Adv Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis Authors - Shuwei Hu View Article

Validation Data Gallery

WB analysis of mouse kidney using 21324-1-AP

WB analysis of HeLa using 21324-1-AP

WB analysis of HEK-293 using 21324-1-AP

WB analysis of mouse brain using 21324-1-AP

IP experiment of HEK-293 using 21324-1-AP

IHC staining of human kidney using 21324-1-AP

IHC staining of human kidney using 21324-1-AP

IF Staining of HeLa using 21324-1-AP

IF Staining of HeLa using 21324-1-AP

IF Staining of HT-29 using 21324-1-AP

FC experiment of HeLa using 21324-1-AP

ZDHHC5 Polyclonal antibody

ZDHHC5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D

Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy

ZDHHC2-mediated AGK palmitoylation activates AKT/mTOR signaling to reduce sunitinib sensitivity in renal cell carcinoma

Neonatal sevoflurane exposures inhibits DHHC5-mediated palmitoylation of TfR1 in oligodendrocytes, leading to hypomyelination and neurological impairments

ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis

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