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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZCCHC6 Polyclonal antibody (25196-1-AP)

    WB analysis of PC-3 using 25196-1-AP (same clone as 25196-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZCCHC6 Polyclonal antibody (25196-1-AP)

    WB analysis using 25196-1-AP (same clone as 25196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ZCCHC6 Polyclonal antibody (25196-1-AP)

    WB analysis of mouse brain using 25196-1-AP (same clone as 25196-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25196-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZCCHC6 Polyclonal antibody (25196-1-AP)

    IP experiment of HeLa using 25196-1-AP (same clone as 25196-1-PBS)

    IP result of anti-ZCCHC6 (IP:25196-1-AP, 4ug; Detection:25196-1-AP 1:1000) with HeLa cells lysate 1800ug. This data was developed using the same antibody clone with 25196-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZCCHC6 Polyclonal antibody, PBS Only

ZCCHC6 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25196-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25196-1-PBS targets ZCCHC6 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18240

Product name: Recombinant human ZCCHC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC032456

Sequence: MGDTAKPYFVKRTKDRGTMDDDDFRRGHPQQDYLIIDDHAKGHGSKMEKGLQKKKITPGNYGNTPRKGPCAVSSNPYAFKNPIYSQPAWMNDSHKDQSKRWLSDEHTGNSDNWREFKPGPRIPVINRQRKDSFQENEDGYRWQDTRGCRTVRRLFHKDLTSLETTSEMEAGSPENKKQRSRPRKPRKTRNEENEQDGDLEGPVIDESVLSTKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCRLCDV

相同性解析による交差性が予測される生物種
Full Name zinc finger, CCHC domain containing 6
Calculated molecular weight 1495 aa, 171 kDa
Observed molecular weight 171 kDa
GenBank accession numberBC032456
Gene Symbol ZCCHC6
Gene ID (NCBI) 79670
RRIDAB_2879953
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VYS8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

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