Validation Data Gallery

Filter: AllWBIP

WB analysis using 25196-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25196-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 25196-1-AP
IP result of anti-ZCCHC6 (IP:25196-1-AP, 4ug; Detection:25196-1-AP 1:1000) with HeLa cells lysate 1800ug.

Featured Product
KD/KO Validated

ZCCHC6 Polyclonal antibody

ZCCHC6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 25196-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ELISA

Synonyms

EC:2.7.7.52, HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, mouse brain tissue, HeLa cells, MCF-7 cells, PC-3 cells
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 14 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

25196-1-AP targets ZCCHC6 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18240 Product name: Recombinant human ZCCHC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC032456 Sequence: MGDTAKPYFVKRTKDRGTMDDDDFRRGHPQQDYLIIDDHAKGHGSKMEKGLQKKKITPGNYGNTPRKGPCAVSSNPYAFKNPIYSQPAWMNDSHKDQSKRWLSDEHTGNSDNWREFKPGPRIPVINRQRKDSFQENEDGYRWQDTRGCRTVRRLFHKDLTSLETTSEMEAGSPENKKQRSRPRKPRKTRNEENEQDGDLEGPVIDESVLSTKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCRLCDV 相同性解析による交差性が予測される生物種
Full Name	zinc finger, CCHC domain containing 6
Calculated molecular weight	1495 aa, 171 kDa
Observed molecular weight	171 kDa
GenBank accession number	BC032456
Gene Symbol	ZCCHC6
Gene ID (NCBI)	79670
RRID	AB_2879953
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5VYS8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

Protocols

Product Specific Protocols
IP protocol for ZCCHC6 antibody 25196-1-AP	Download protocol
WB protocol for ZCCHC6 antibody 25196-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2. Authors - Acong Yang View Article
human	WB,IP	Nat Commun TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance Authors - Acong Yang View Article
human	IF	Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. Authors - Hagai Marmor-Kollet View Article
human	WB	Mol Cell 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires. Authors - Acong Yang KO Validated View Article
human	WB	Mol Cell A Mechanism for microRNA Arm Switching Regulated by Uridylation. Authors - Haedong Kim KD Validated View Article
human	WB	Cell Rep Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells. Authors - Kazutoshi Takahashi View Article

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Validation Data Gallery

WB analysis using 25196-1-AP

WB analysis of mouse brain using 25196-1-AP

IP experiment of HeLa using 25196-1-AP

ZCCHC6 Polyclonal antibody

ZCCHC6 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.

A Mechanism for microRNA Arm Switching Regulated by Uridylation.

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.

Able^™

Validation Data Gallery

WB analysis using 25196-1-AP

WB analysis of mouse brain using 25196-1-AP

IP experiment of HeLa using 25196-1-AP

ZCCHC6 Polyclonal antibody

ZCCHC6 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.

A Mechanism for microRNA Arm Switching Regulated by Uridylation.

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.

Able™

Able^™