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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse testis using 25631-1-AP (same clone as 25631-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse testis using 25631-1-AP (same clone as 25631-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse spleen using 25631-1-AP (same clone as 25631-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZBTB25 Polyclonal antibody (25631-1-AP)

    IF Staining of HepG2 using 25631-1-AP (same clone as 25631-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25631-1-AP (ZBTB25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25631-1-PBS in a different storage buffer formulation.

ZBTB25 Polyclonal antibody, PBS Only

ZBTB25 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25631-1-PBS targets ZBTB25 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22507

Product name: Recombinant human ZBTB25 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 154-435 aa of BC035804

Sequence: NSGNRAAVQGDHPQLQLSLAIGLDDGTADQQRACPATQALEEHQKPPVSIKQERCDPESVISQSHPSPSSEVTGPTFTENSVKIHLCHYCGERFDSRSNLRQHLHTHVSGSLPFGVPASILESNDLGEVHPLNENSEALECRRLSSFIVKENEQQPDHTNRGTTEPLQISQVSLISKDTEPVELNCNFSFSRKRKMSCTICGHKFPRKSQLLEHMYTHKGKSYRYNRCQRFGNALAQRFQPYCDSWSDVSLKSSRLSQEHLDLPCALESELTQENVDTILVE

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 25
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC035804
Gene Symbol ZBTB25
Gene ID (NCBI) 7597
RRIDAB_2880171
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP24278
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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