• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZBTB25 Polyclonal antibody

ZBTB25 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25631-1-AP

Synonyms

C14orf51, KUP, ZBTB25, Zinc finger protein 46, Zinc finger protein KUP, ZNF46

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 25631-1-AP

    WB analysis of mouse testis using 25631-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis of mouse testis using 25631-1-AP

    WB analysis of mouse testis using 25631-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 25631-1-AP

    WB analysis of mouse spleen using 25631-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of HepG2 using 25631-1-AP

    IF Staining of HepG2 using 25631-1-AP

    Immunofluorescent analysis of HepG2 cells using 25631-1-AP (ZBTB25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, mouse spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25631-1-AP is ZBTB25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB25 fusion protein Ag22507 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 25
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC035804
Gene symbol ZBTB25
Gene ID (NCBI) 7597
RRIDAB_2880171
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZBTB25 antibody 25631-1-APDownload protocol
IF protocol for ZBTB25 antibody 25631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article