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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using ZBTB10 Polyclonal antibody (22944-1-AP)

    WB analysis of A375 using 22944-1-AP (same clone as 22944-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 22944-1-AP (ZBTB10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22944-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ZBTB10 Polyclonal antibody (22944-1-AP)

    IP experiment of mouse liver using 22944-1-AP (same clone as 22944-1-PBS)

    IP result of anti-ZBTB10 (IP:22944-1-AP, 3ug; Detection:22944-1-AP 1:1000) with mouse liver tissue lysate 12500ug. This data was developed using the same antibody clone with 22944-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using ZBTB10 Polyclonal antibody (22944-1-AP)

    IP experiment using 22944-1-AP (same clone as 22944-1-PBS)

    IP result of ZBTB10 antibody (22944-1-AP for IP and Detection) with mouse liver tissue lysate. This data was developed using the same antibody clone with 22944-1-PBS in a different storage buffer formulation.

ZBTB10 Polyclonal antibody, PBS Only

ZBTB10 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 22944-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22944-1-PBS targets ZBTB10 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19094

Product name: Recombinant human ZBTB10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 299-519 aa of BC127098

Sequence: PESVVVDYNNRKPVNRDGLSSSRDQKIASFWATRNLTNLASNVKIENDGCNVDEGQIENYQMNDSSWVQDGSPEMAENESEGQTKVFIWNNMGSQGIQETGKTRRKNQTTKRFIYNIPPNNETNLEDCSVMQPPVAYPEENTLLIKEEPDLDGALLSGPDGDRNVNANLLAEAGTSQDGGDAGTSHDFKYGLMPGPSNDFKYGLIPGTSNDFKYGLIPGAS

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 10
Calculated molecular weight 871 aa, 95 kDa
Observed molecular weight 65 kDa, 130 kDa
GenBank accession numberBC127098
Gene Symbol ZBTB10
Gene ID (NCBI) 65986
RRIDAB_2879188
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96DT7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc finger and BTB domain-containing protein 10 (ZBTB10), also known as RINZF, RINZFC, May be involved in transcriptional regulation. Multiple isoforms of ZBTB10 exist due to alternative splicing events.

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