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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZBTB10 Polyclonal antibody

ZBTB10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22944-1-AP

Synonyms

RINZF, RINZFC, ZBTB10, Zinc finger protein RIN ZF

Validation Data Gallery

Applications:
  • WB analysis of A375 using 22944-1-AP

    WB analysis of A375 using 22944-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 22944-1-AP (ZBTB10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 22944-1-AP

    IP experiment of mouse liver using 22944-1-AP

    IP result of anti-ZBTB10 (IP:22944-1-AP, 3ug; Detection:22944-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

  • IP experiment using 22944-1-AP

    IP experiment using 22944-1-AP

    IP result of ZBTB10 antibody (22944-1-AP for IP and Detection) with mouse liver tissue lysate.

Tested Applications

Positive WB detected inA375 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22944-1-AP is ZBTB10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB10 fusion protein Ag19094 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 10
Calculated molecular weight 871 aa, 95 kDa
Observed molecular weight 65 kDa, 130 kDa
GenBank accession numberBC127098
Gene symbol ZBTB10
Gene ID (NCBI) 65986
RRIDAB_2879188
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger and BTB domain-containing protein 10 (ZBTB10), also known as RINZF, RINZFC, May be involved in transcriptional regulation. Multiple isoforms of ZBTB10 exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for ZBTB10 antibody 22944-1-APDownload protocol
IP protocol for ZBTB10 antibody 22944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols