Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-25537 targets YTHDF3-specific in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22374 Product name: Recombinant human YTHDF3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-351 aa of BC052970 Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ 相同性解析による交差性が予測される生物種 |
| Full Name | YTH domain family, member 3 |
| Calculated molecular weight | 585 aa, 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC052970 |
| Gene Symbol | YTHDF3 |
| Gene ID (NCBI) | 253943 |
| RRID | AB_3672794 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z739 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 YTHDF3-specific antibody CL488-25537 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |