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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using YIPF4 Polyclonal antibody (15473-1-PBS)

    WB analysis of HeLa using 15473-1-PBS

    HeLa cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP (same clone as 15473-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using YIPF4 Polyclonal antibody (15473-1-PBS)

    WB analysis of Jurkat using 15473-1-PBS

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

YIPF4 Polyclonal antibody, PBS Only

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15473-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15473-1-PBS targets YIPF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7777

Product name: Recombinant human YIPF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC004875

Sequence: MQPPGPPPAYAPTNGDFTFVSSADAEDLSGSIASPDVKLNLGGDFIKESTATTFLRQRGYGWLLEVEDDDPEDNKPLLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNP

相同性解析による交差性が予測される生物種
Full Name Yip1 domain family, member 4
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC004875
Gene Symbol YIPF4
Gene ID (NCBI) 84272
RRIDAB_2217206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSR8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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