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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin.

YIPF4 Polyclonal antibody

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15473-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, HaCaT cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15473-1-AP targets YIPF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7777

Product name: Recombinant human YIPF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC004875

Sequence: MQPPGPPPAYAPTNGDFTFVSSADAEDLSGSIASPDVKLNLGGDFIKESTATTFLRQRGYGWLLEVEDDDPEDNKPLLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNP

相同性解析による交差性が予測される生物種
Full Name Yip1 domain family, member 4
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC004875
Gene Symbol YIPF4
Gene ID (NCBI) 84272
RRIDAB_2217206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for YIPF4 antibody 15473-1-APDownload protocol
IHC protocol for YIPF4 antibody 15473-1-APDownload protocol
WB protocol for YIPF4 antibody 15473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Blocking Lysine Crotonylation and Aerobic Glycolysis as Targeting Strategy Against mpox Virus Replication.

Authors - Pengjun Wei
View Article
humanWB

Nat Struct Mol Biol

DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation

Authors - Chan Lee
View Article

Dev Cell

TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy

Authors - Jiejie Yang
View Article
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