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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WWOX Polyclonal antibody (15299-1-AP)

    WB analysis using 15299-1-AP (same clone as 15299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using WWOX Polyclonal antibody (15299-1-AP)

    WB analysis of MCF-7 using 15299-1-AP (same clone as 15299-1-PBS)

    WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using WWOX Polyclonal antibody (15299-1-AP)

    WB analysis of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using WWOX Polyclonal antibody (15299-1-AP)

    IP experiment of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

    IP result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WWOX Polyclonal antibody (15299-1-AP)

    IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WWOX Polyclonal antibody, PBS Only

WWOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15299-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15299-1-PBS targets WWOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7555

Product name: Recombinant human WWOX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC003184

Sequence: MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG

相同性解析による交差性が予測される生物種
Full Name WW domain containing oxidoreductase
Calculated molecular weight 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC003184
Gene Symbol WWOX
Gene ID (NCBI) 51741
RRIDAB_2216389
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZC7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

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