Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15299-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15299-1-AP
WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells.
WB analysis of mouse skeletal muscle using 15299-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 15299-1-AP
IP result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.
IHC staining of human liver cancer using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 15299-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

WWOX Polyclonal antibody

WWOX Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15299-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human liver cancer tissue, mouse liver tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

15299-1-AP targets WWOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7555 Product name: Recombinant human WWOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC003184 Sequence: MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG 相同性解析による交差性が予測される生物種
Full Name	WW domain containing oxidoreductase
Calculated molecular weight	47 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC003184
Gene Symbol	WWOX
Gene ID (NCBI)	51741
RRID	AB_2216389
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZC7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IHC protocol for WWOX antibody 15299-1-AP	Download protocol
IP protocol for WWOX antibody 15299-1-AP	Download protocol
WB protocol for WWOX antibody 15299-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Hum Mutat Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy. Authors - Mariska Davids View Article
human	WB,IHC	Front Oncol WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma Authors - Yu-De Chu KD Validated View Article
human	WB	Molecules Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway. Authors - Che-Yuan Hu View Article
human	WB	Int J Mol Med WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines. Authors - Qu Junjie J KD Validated View Article
human	IHC	Oncol Lett Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer. Authors - Yakun Hu View Article
human	WB	Int J Cancer Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients Authors - Lei Cheng KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15299-1-AP

WB analysis of MCF-7 using 15299-1-AP

WB analysis of mouse skeletal muscle using 15299-1-AP

IP experiment of mouse skeletal muscle using 15299-1-AP

IHC staining of human liver cancer using 15299-1-AP

IHC staining of human liver cancer using 15299-1-AP

IHC staining of human breast cancer using 15299-1-AP

IHC staining of human breast cancer using 15299-1-AP

IHC staining of mouse liver using 15299-1-AP

IHC staining of mouse liver using 15299-1-AP

WWOX Polyclonal antibody

WWOX Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy.

WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma

Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway.

WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.

Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer.

Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients