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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT3 Polyclonal antibody (28156-1-AP)

    WB analysis using 28156-1-AP (same clone as 28156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WNT3 Polyclonal antibody (28156-1-AP)

    IHC staining of mouse brain using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using WNT3 Polyclonal antibody (28156-1-AP)

    IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using WNT3 Polyclonal antibody (28156-1-AP)

    IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using WNT3 Polyclonal antibody (28156-1-AP)

    IHC staining of mouse kidney using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

WNT3 Polyclonal antibody, PBS Only

WNT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28156-1-PBS targets WNT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27965

Product name: Recombinant human WNT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-306 aa of BC114219

Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG

相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 3
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC114219
Gene Symbol WNT3
Gene ID (NCBI) 7473
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP56703
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).

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