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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT3 Polyclonal antibody

WNT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28156-1-AP

Synonyms

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3

Validation Data Gallery

Applications:
  • WB analysis using 28156-1-AP

    WB analysis using 28156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28156-1-AP

    IHC staining of mouse brain using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 28156-1-AP

    IHC staining of mouse colon using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 28156-1-AP

    IHC staining of mouse colon using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28156-1-AP

    IHC staining of mouse kidney using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHCT 116 cells, MCF-7 cells
Positive IHC detected inmouse colon tissue, mouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28156-1-AP targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT3 fusion protein Ag27965 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 3
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC114219
Gene symbol WNT3
Gene ID (NCBI) 7473
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).

Protocols

Product Specific Protocols
WB protocol for WNT3 antibody 28156-1-APDownload protocol
IHC protocol for WNT3 antibody 28156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols