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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated WNT2 Monoclonal antibody (CL594-66656)

    IF Staining of MCF-7 using CL594-66656

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 WNT2 antibody (CL594-66656, Clone: 3H7B10 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite®594-conjugated WNT2 Monoclonal antibody

WNT2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66656
Clone No.3H7B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

3H7B10, Int 1 like protein 1, Int 1 related protein, INT1L1, Int-1-like protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66656 targets WNT2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WNT2 fusion protein Ag25185 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family member 2
Calculated molecular weight 40 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC029854
Gene Symbol WNT2
Gene ID (NCBI) 7472
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09544
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Protocols

Product Specific Protocols
IF protocol for CL594 WNT2 antibody CL594-66656Download protocol
Standard Protocols
Click here to view our Standard Protocols