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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT10B Monoclonal antibody (67210-1-Ig)

    WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WNT10B Monoclonal antibody (67210-1-Ig)

    IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WNT10B Monoclonal antibody, PBS Only

WNT10B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67210-1-PBS
Clone No.1G11E4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1G11E4, Protein Wnt 10b, Protein Wnt 12, Protein Wnt-10b, Protein Wnt-12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67210-1-PBS targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17114

Product name: Recombinant human WNT10B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 19-204 aa of BC096353

Sequence: FLALCSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRNPDVTASALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQDRLRAKLLQLQALSRGKSFPHSLPSPGPGSSPSPGPQDTWEWGGCNHDMD

相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 10B
Calculated molecular weight 389 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC096353
Gene Symbol WNT10B
Gene ID (NCBI) 7480
RRIDAB_2882503
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO00744
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.