Validation Data Gallery
Tested Applications
| Positive WB detected in | NCCIT cells, MCF-7 cells, NCI-H1299 cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | mouse liver tissue, mouse skeletal muscle tissue, human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
67210-1-Ig targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17114 Product name: Recombinant human WNT10B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-204 aa of BC096353 Sequence: FLALCSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRNPDVTASALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQDRLRAKLLQLQALSRGKSFPHSLPSPGPGSSPSPGPQDTWEWGGCNHDMD 相同性解析による交差性が予測される生物種 |
| Full Name | wingless-type MMTV integration site family, member 10B |
| Calculated molecular weight | 389 aa, 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC096353 |
| Gene Symbol | WNT10B |
| Gene ID (NCBI) | 7480 |
| RRID | AB_2882503 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O00744 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| IHC protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| IF protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Res Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.
| ||
J Cancer MPP7 mediates EMT via Wnt/β-catenin pathway to promote polarity changes in epithelial ovarian cancer cells | ||
Nat Commun FoxO3 controls cardiomyocyte proliferation and heart regeneration by regulating Sfrp2 expression in postnatal mice | ||
Int J Biol Macromol Xylooligosaccharides alleviate the carbohydrate-enriched diet-induced intestinal barrier dysfunction in carp Megalobrama amblycephala by promoting intestinal development, immunity and gut microbiota | ||
Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling |