Validation Data Gallery
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Tested Applications
| Positive WB detected in | Caco-2 cells, SW480 cells, COLO 320 cells, HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67904-1-Ig targets WIF1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23972 Product name: Recombinant human WIF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC018037 Sequence: MARRSAFPAAALWLWSILLCLLALRAEAGPPQEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQAAGQAEYFYEFLSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILKTPQNAIFFKTCQQA 相同性解析による交差性が予測される生物種 |
| Full Name | WNT inhibitory factor 1 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC018037 |
| Gene Symbol | WIF1 |
| Gene ID (NCBI) | 11197 |
| RRID | AB_2918660 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y5W5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WIF1 antibody 67904-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway | ||
Aging (Albany NY) Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma |