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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis using 20631-1-AP (same clone as 20631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of HepG2 using 20631-1-AP (same clone as 20631-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of K-562 using 20631-1-AP (same clone as 20631-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of MCF-7 using 20631-1-AP (same clone as 20631-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocellular carcinoma using WDR74 Polyclonal antibody (20631-1-AP)

    IHC staining of human hepatocellular carcinoma using 20631-1-AP (same clone as 20631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 20631-1-AP (WDR74 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

WDR74 Polyclonal antibody, PBS Only

WDR74 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

NOP seven associated protein 1, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20631-1-PBS targets WDR74 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14617

Product name: Recombinant human WDR74 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC006351

Sequence: MAAAAARWNHVWVGTETGILKGVNLQRKQAANFTAGGQPRREEAVSALCWGTGGETQMLVGCADRTVKHFSTEDGIFQGQRHCPGGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDPAHPHVVATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVYDPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQCHPSKPLLASCGLDRVLRIHRIQN

相同性解析による交差性が予測される生物種
Full Name WD repeat domain 74
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC006351
Gene Symbol WDR74
Gene ID (NCBI) 54663
RRIDAB_2878713
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6RFH5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.

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