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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis using 20631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of MCF-7 using 20631-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of HepG2 using 20631-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of K-562 using 20631-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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WDR74 Polyclonal antibody

WDR74 Polyclonal Antibody for WB, ELISA
Cat No. 20631-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NOP seven associated protein 1, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells, L02 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20631-1-AP targets WDR74 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR74 fusion protein Ag14617 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 74
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC006351
Gene Symbol WDR74
Gene ID (NCBI) 54663
RRIDAB_2878713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6RFH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.

Protocols

Product Specific Protocols
WB protocol for WDR74 antibody 20631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols