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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR43 Polyclonal antibody (30227-1-AP)

    WB analysis using 30227-1-AP (same clone as 30227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30227-1-AP (WDR43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using WDR43 Polyclonal antibody (30227-1-AP)

    IHC staining of mouse lung using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using WDR43 Polyclonal antibody (30227-1-AP)

    IHC staining of mouse ovary using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using WDR43 Polyclonal antibody (30227-1-AP)

    IHC staining of rat liver using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat ovary tissue using WDR43 Polyclonal antibody (30227-1-AP)

    IHC staining of rat ovary using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WDR43 Polyclonal antibody (30227-1-AP)

    IF Staining of HeLa using 30227-1-AP (same clone as 30227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR43 antibody (30227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

WDR43 Polyclonal antibody, PBS Only

WDR43 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30227-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0007, WD repeat domain 43

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30227-1-PBS targets WDR43 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30585

Product name: Recombinant human WDR43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-150 aa of NM_015131

Sequence: MAAGGGGSCDPLAPAGVPCAFSPHSQAYFALASTDGHLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPARLQAKESPQRKKRKSEAVGMSNQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGCLYSCSDDKHI

相同性解析による交差性が予測される生物種
Full Name WD repeat domain 43
Calculated molecular weight 75 kDa
Observed molecular weight75 kDa
GenBank accession numberNM_015131
Gene Symbol WDR43
Gene ID (NCBI) 23160
RRIDAB_3086268
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15061
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)

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