Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | mouse lung tissue, mouse ovary tissue, rat liver tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
30227-1-AP targets WDR43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30585 Product name: Recombinant human WDR43 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-150 aa of NM_015131 Sequence: MAAGGGGSCDPLAPAGVPCAFSPHSQAYFALASTDGHLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPARLQAKESPQRKKRKSEAVGMSNQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGCLYSCSDDKHI 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 43 |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | NM_015131 |
| Gene Symbol | WDR43 |
| Gene ID (NCBI) | 23160 |
| RRID | AB_3086268 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WDR43 antibody 30227-1-AP | Download protocol |
| IHC protocol for WDR43 antibody 30227-1-AP | Download protocol |
| WB protocol for WDR43 antibody 30227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation | ||
Sci Adv Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib. |