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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Villin like Polyclonal antibody (15130-1-AP)

    WB analysis using 15130-1-AP (same clone as 15130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15130-1-AP (VILL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IHC staining of mouse small intestine using 15130-1-AP (same clone as 15130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15130-1-AP (VILL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of mouse small intestine using 15130-1-AP (same clone as 15130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of rat small intestine using 15130-1-AP (same clone as 15130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

Villin like Polyclonal antibody, PBS Only

Villin like Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 15130-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15130-1-PBS targets Villin like in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7259

Product name: Recombinant human VILL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-687 aa of BC000243

Sequence: SASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQREMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVGFFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIGWFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDLVRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYSMATWRQRQEKKQLGFF

相同性解析による交差性が予測される生物種
Full Name villin-like
Calculated molecular weight 96 kDa
Observed molecular weight96-100 kDa
GenBank accession numberBC000243
Gene Symbol VILL
Gene ID (NCBI) 50853
RRIDAB_2918038
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15195
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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