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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Villin like Polyclonal antibody (15130-1-AP)

    WB analysis using 15130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15130-1-AP (VILL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IHC staining of mouse small intestine using 15130-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15130-1-AP (VILL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of mouse small intestine using 15130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of rat small intestine using 15130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Villin like Polyclonal antibody

Villin like Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 15130-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

VILL, Villin-like protein, Villin like protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in37°C incubated mouse colon tissue, rat colon
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, rat small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15130-1-AP targets Villin like in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7259

Product name: Recombinant human VILL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-687 aa of BC000243

Sequence: SASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQREMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVGFFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIGWFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDLVRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYSMATWRQRQEKKQLGFF

相同性解析による交差性が予測される生物種
Full Name villin-like
Calculated molecular weight 96 kDa
Observed molecular weight96-100 kDa
GenBank accession numberBC000243
Gene Symbol VILL
Gene ID (NCBI) 50853
RRIDAB_2918038
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15195
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Villin like antibody 15130-1-APDownload protocol
IHC protocol for Villin like antibody 15130-1-APDownload protocol
WB protocol for Villin like antibody 15130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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