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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Villin Polyclonal antibody

Villin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16488

Synonyms

D2S1471, VIL, VIL1, villin 1

Validation Data Gallery

Applications:
  • IF Staining of mouse small intestine using CL488-16488

    IF Staining of mouse small intestine using CL488-16488

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CoraLite® Plus 488 Villin antibody (CL488-16488) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16488-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-16488 targets Villin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Villin fusion protein Ag9610 相同性解析による交差性が予測される生物種
Full Name villin 1
Calculated molecular weight827aa,93 kDa; 826aa,93 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC017303
Gene symbol VIL1
Gene ID (NCBI) 7429
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Villin antibody CL488-16488Download protocol
Standard Protocols
Click here to view our Standard Protocols