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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS33B Polyclonal antibody (28653-1-AP)

    WB analysis using 28653-1-AP (same clone as 28653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28653-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP (same clone as 28653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP (same clone as 28653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP (same clone as 28653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP (same clone as 28653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

VPS33B Polyclonal antibody, PBS Only

VPS33B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28653-1-PBS targets VPS33B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29886

Product name: Recombinant human VPS33B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-508 aa of BC016445

Sequence: EGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRD

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated molecular weight 617 aa, 71 kDa
Observed molecular weight65 kDa
GenBank accession numberBC016445
Gene Symbol VPS33B
Gene ID (NCBI) 26276
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H267
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

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