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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS33B Polyclonal antibody (28653-1-AP)

    WB analysis using 28653-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28653-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VPS33B Polyclonal antibody

VPS33B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28653-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FLJ14848, hVPS33B, VPS33B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28653-1-AP targets VPS33B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29886

Product name: Recombinant human VPS33B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-508 aa of BC016445

Sequence: EGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRD

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated molecular weight 617 aa, 71 kDa
Observed molecular weight65 kDa
GenBank accession numberBC016445
Gene Symbol VPS33B
Gene ID (NCBI) 26276
RRIDAB_3086076
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H267
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Protocols

Product Specific Protocols
WB protocol for VPS33B antibody 28653-1-APDownload protocol
IHC protocol for VPS33B antibody 28653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols