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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using VPS28 Polyclonal antibody (15478-1-AP)

    WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using VPS28 Polyclonal antibody (15478-1-AP)

    WB analysis of HeLa using 15478-1-AP (same clone as 15478-1-PBS)

    WB result of VPS28 antibody (15478-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS28 Polyclonal antibody (15478-1-AP)

    WB analysis using 15478-1-AP (same clone as 15478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VPS28 Polyclonal antibody (15478-1-AP)

    IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS28 Polyclonal antibody (15478-1-AP)

    IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15478-1-PBS targets VPS28 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS28 fusion protein Ag7784 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
RRIDAB_2878144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK41
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.