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  • KD/KO Validated

VPS28 Polyclonal antibody

VPS28 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15478-1-AP

Synonyms

ESCRT I complex subunit VPS28, H Vps28, VPS28



Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15478-1-AP targets VPS28 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS28 fusion protein Ag7784 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC006485
Gene symbol VPS28
Gene ID (NCBI) 51160
RRIDAB_2878144
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for VPS28 antibody 15478-1-APDownload protocol
IF protocol for VPS28 antibody 15478-1-APDownload protocol
IP protocol for VPS28 antibody 15478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS Genet

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

Authors - Chiara Merigliano
humanWB,IF

iScience

VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.

Authors - Xiaohua Dong
  • KD Validated
humanWB

Curr Protoc

Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids

Authors - Emilie Rigaux
humanWB,IF

Nat Commun

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy

Authors - Takashi Nozawa
  • KD Validated