Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, Jurkat cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
Product Information
15478-1-AP targets VPS28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7784 Product name: Recombinant human VPS28 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-221 aa of BC006485 Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 28 homolog (S. cerevisiae) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC006485 |
| Gene Symbol | VPS28 |
| Gene ID (NCBI) | 51160 |
| RRID | AB_2878144 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IHC protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IF protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IP protocol for VPS28 antibody 15478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody. | ||
iScience VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.
| ||
Curr Protoc Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids | ||
J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer | ||
Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy
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