Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL532-83919-4 targets VISTA in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0017 Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 33-194 aa of BC020568 Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 10 open reading frame 54 |
| Calculated molecular weight | 311 aa, 34 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC020568 |
| Gene Symbol | VISTA |
| Gene ID (NCBI) | 64115 |
| Conjugate | CoraLite®532 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 537 nm / 560 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H7M9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL532 VISTA antibody CL532-83919-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
