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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated VISTA Polyclonal antibody

VISTA Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24849

Synonyms

B7-H5, C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412, VISTA, VSIR

Validation Data Gallery

Applications:
  • IF Staining of human placenta using CL488-24849

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using CL488-24849

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inhuman placenta tissue
For other applications, we recommend the unconjugated version of this antibody, 24849-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-24849 targets VISTA in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VISTA fusion protein Ag20600 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight45-60 kDa
GenBank accession numberBC020568
Gene symbol C10orf54
Gene ID (NCBI) 64115
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VISTA antibody CL488-24849Download protocol
Standard Protocols
Click here to view our Standard Protocols