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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55491

Synonyms

BNPI, SLC17A7, VGLUT1

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL488-55491

    IF Staining of mouse brain using CL488-55491

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL488-55491

    FC experiment of SH-SY5Y using CL488-55491

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 55491-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-55491 targets VGLUT1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Calculated molecular weight 62 kDa
GenBank accession numberNM_020309
Gene symbol SLC17A7
Gene ID (NCBI) 57030
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
FC protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
Standard Protocols
Click here to view our Standard Protocols