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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated VDAC1/2 Polyclonal antibody

VDAC1/2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10866

Synonyms

hVDAC1, Plasmalemmal porin, PORIN, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1

Validation Data Gallery

Applications:
  • IF Staining of mouse liver using CL488-10866

    IF Staining of mouse liver using CL488-10866

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 VDAC1/2 antibody (CL488-10866) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10866-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10866 targets VDAC1/2 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VDAC1/2 fusion protein Ag1144 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC008482
Gene symbol VDAC1
Gene ID (NCBI) 7416
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VDAC1/2 antibody CL488-10866Download protocol
Standard Protocols
Click here to view our Standard Protocols