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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VASH2 Monoclonal antibody (67753-1-Ig)

    WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VASH2 Monoclonal antibody (67753-1-Ig)

    WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Monoclonal antibody (67753-1-Ig)

    IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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VASH2 Monoclonal antibody, PBS Only

VASH2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67753-1-PBS
Clone No.3A11D10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67753-1-PBS targets VASH2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VASH2 fusion protein Ag29985 相同性解析による交差性が予測される生物種
Full Name vasohibin 2
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight30-34 kDa
GenBank accession numberBC028194
Gene Symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_2918521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86V25
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)