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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VASH2 Monoclonal antibody

VASH2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A11D10

Cat no : 67753-1-Ig

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

Validation Data Gallery

Applications:
  • WB analysis using 67753-1-Ig

    WB analysis using 67753-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 67753-1-Ig

    WB analysis using 67753-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67753-1-Ig

    IHC staining of human skin cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 67753-1-Ig

    IHC staining of human skin cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67753-1-Ig

    IHC staining of human liver cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67753-1-Ig

    IHC staining of human liver cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67753-1-Ig

    IHC staining of human liver cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67753-1-Ig

    IHC staining of human liver cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSKOV-3 cells, BGC-823 cells, L02 cells, HepG2 cells, HUVEC cells, HSC-T6 cells, NIH/3T3 cells, SGC-7901 cells, MKN-45 cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67753-1-Ig targets VASH2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VASH2 fusion protein Ag29985 相同性解析による交差性が予測される生物種
Full Name vasohibin 2
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight34 kDa
GenBank accession numberBC028194
Gene symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_2918521
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)

Protocols

Product Specific Protocols
WB protocol for VASH2 antibody 67753-1-IgDownload protocol
IHC protocol for VASH2 antibody 67753-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols