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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated VAMP4 Monoclonal ant (CL488-67219)

    IF Staining of HEK-293 using CL488-67219

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite® Plus 488 VAMP4 antibody (CL488-67219, Clone: 3A6F2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for IF/ICC
Cat No. CL488-67219
Clone No.3A6F2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse

Applications

IF/ICC

Synonyms

VAMP 4, VAMP24, VAMP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67219 targets VAMP4 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Reactivity Human, Pig, Rat, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27854

Product name: Recombinant human VAMP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC007019

Sequence: MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 4
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC007019
Gene Symbol VAMP4
Gene ID (NCBI) 8674
RRIDAB_2919438
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75379
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VAMP4 antibody CL488-67219Download protocol
Standard Protocols
Click here to view our Standard Protocols