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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using V5-tag Recombinant antibody (81775-4-RR)

    WB analysis using 81775-4-RR

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using V5-tag Recombinant antibody (81775-4-RR)

    WB analysis of Recombinant protein using 81775-4-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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V5-tag Recombinant antibody

V5-tag Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81775-4-RR
Clone No.240529D8

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Synonyms

V5 tag, 240529D8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTransfected HEK-293 cells, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81775-4-RR targets V5-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name V5 Tag
Calculated molecular weight 1 kDa
Gene Symbol V5 Tag
Gene ID (NCBI) 4
RRIDAB_3670504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Protocols

Product Specific Protocols
WB protocol for V5-tag antibody 81775-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols