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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using V5-tag Recombinant antibody (81775-4-RR)

    WB analysis using 81775-4-RR (same clone as 81775-4-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81775-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using V5-tag Recombinant antibody (81775-4-RR)

    WB analysis of Recombinant protein using 81775-4-RR (same clone as 81775-4-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81775-4-PBS in a different storage buffer formulation.

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V5-tag Recombinant antibody, PBS Only

V5-tag Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81775-4-PBS
Clone No.240529D8

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Synonyms

240529D8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81775-4-PBS targets V5-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name V5 Tag
Calculated molecular weight 1 kDa
Gene Symbol V5 Tag
Gene ID (NCBI) 4
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.