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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis using 19731-1-AP (same clone as 19731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of HeLa using 19731-1-AP (same clone as 19731-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of HEK-293 using 19731-1-AP (same clone as 19731-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of human kidney using 19731-1-AP (same clone as 19731-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of human testis using 19731-1-AP (same clone as 19731-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using Urocortin Polyclonal antibody (19731-1-AP)

    IP experiment of mouse kidney using 19731-1-AP (same clone as 19731-1-PBS)

    IP result of anti-Urocortin (IP:19731-1-AP, 4ug; Detection:19731-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 19731-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19731-1-PBS targets Urocortin in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name urocortin
Calculated molecular weight 13 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_003353
Gene Symbol UCN
Gene ID (NCBI) 7349
RRIDAB_10640909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55089
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).