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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis of HEK-293 using 23865-1-AP (same clone as 23865-1-PBS)

    WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis of HeLa using 23865-1-AP (same clone as 23865-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using USP39 Polyclonal antibody (23865-1-AP)

    WB analysis of HepG2 using 23865-1-AP (same clone as 23865-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using USP39 Polyclonal antibody (23865-1-AP)

    IP experiment of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP39 Polyclonal antibody (23865-1-AP)

    IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP39 Polyclonal antibody (23865-1-AP)

    IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP39 Polyclonal antibody (23865-1-AP)

    IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using USP39 Polyclonal antibody (23865-1-AP)

    IF Staining of A431 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using USP39 Polyclonal antibody (23865-1-AP)

    IF Staining of A549 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using USP39 Polyclonal antibody (23865-1-AP)

    IF Staining of MCF-7 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP39 Polyclonal antibody, PBS Only

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23865-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23865-1-PBS targets USP39 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20894

Product name: Recombinant human USP39 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-357 aa of BC067273

Sequence: MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFVRVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFT

相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 39
Calculated molecular weight 565 aa, 65 kDa
Observed molecular weight 60~65 kDa
GenBank accession numberBC067273
Gene Symbol USP39
Gene ID (NCBI) 10713
RRIDAB_2879343
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GS9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2

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