Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of K-562 using 23865-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 23865-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23865-1-AP
WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells.
WB analysis of HepG2 using 23865-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23865-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 23865-1-AP
IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug.
IHC staining of human colon cancer using 23865-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 23865-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 23865-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 23865-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of A549 using 23865-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 23865-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

USP39 Polyclonal antibody

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23865-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, A431 cells, A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 16 publications below
IHC	See 4 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 4 publications below

Product Information

23865-1-AP targets USP39 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20894 Product name: Recombinant human USP39 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-357 aa of BC067273 Sequence: MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFVRVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFT 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 39
Calculated molecular weight	565 aa, 65 kDa
Observed molecular weight	60~65 kDa
GenBank accession number	BC067273
Gene Symbol	USP39
Gene ID (NCBI)	10713
RRID	AB_2879343
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q53GS9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2

Protocols

Product Specific Protocols
IF protocol for USP39 antibody 23865-1-AP	Download protocol
IHC protocol for USP39 antibody 23865-1-AP	Download protocol
IP protocol for USP39 antibody 23865-1-AP	Download protocol
WB protocol for USP39 antibody 23865-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	RIP	Immunity Lactate modulates RNA splicing to promote CTLA-4 expression in tumor-infiltrating regulatory T cells Authors - Rui Ding View Article
human	WB	Sci Adv Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection Authors - Yiyao Huang View Article
mouse,human	RIP,IHC	Mol Oncol Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation. Authors - Yue Xiao KD Validated View Article
human	WB	Biomolecules USP39-Mediated Non-Proteolytic Control of ETS2 Suppresses Nuclear Localization and Activity Authors - Yunsik Choi View Article
human	WB,IF	Am J Cancer Res USP39 facilitates breast cancer cell proliferation through stabilization of FOXM1 Authors - Zhenwang Zhang KD Validated View Article
human	WB,IHC	Oncotarget Overexpression of USP39 predicts poor prognosis and promotes tumorigenesis of prostate cancer via promoting EGFR mRNA maturation and transcription elongation. Authors - Yi Huang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 23865-1-AP

WB analysis using 23865-1-AP

WB analysis of HEK-293 using 23865-1-AP

WB analysis of HepG2 using 23865-1-AP

WB analysis of HeLa using 23865-1-AP

IP experiment of K-562 using 23865-1-AP

IHC staining of human colon cancer using 23865-1-AP

IHC staining of human colon cancer using 23865-1-AP

IHC staining of human colon cancer using 23865-1-AP

IF Staining of A431 using 23865-1-AP

IF Staining of A549 using 23865-1-AP

IF Staining of MCF-7 using 23865-1-AP

USP39 Polyclonal antibody

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lactate modulates RNA splicing to promote CTLA-4 expression in tumor-infiltrating regulatory T cells

Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection

Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation.

USP39-Mediated Non-Proteolytic Control of ETS2 Suppresses Nuclear Localization and Activity

USP39 facilitates breast cancer cell proliferation through stabilization of FOXM1

Overexpression of USP39 predicts poor prognosis and promotes tumorigenesis of prostate cancer via promoting EGFR mRNA maturation and transcription elongation.