Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, mouse liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82964 targets URAT1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag27805 Product name: Recombinant human URAT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-351 aa of BC053348 Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 22 (organic anion/urate transporter), member 12 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC053348 |
| Gene Symbol | URAT1 |
| Gene ID (NCBI) | 116085 |
| RRID | AB_3673754 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96S37 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 URAT1 antibody CL750-82964 | Download protocol |
| WB protocol for CL Plus 750 URAT1 antibody CL750-82964 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
