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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBXN2B Polyclonal antibody (25141-1-AP)

    WB analysis using 25141-1-AP (same clone as 25141-1-PBS)

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UBXN2B Polyclonal antibody (25141-1-AP)

    IHC staining of human ovary cancer using 25141-1-AP (same clone as 25141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using UBXN2B Polyclonal antibody (25141-1-AP)

    IF Staining of Neuro-2a using 25141-1-AP (same clone as 25141-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

UBXN2B Polyclonal antibody, PBS Only

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25141-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25141-1-PBS targets UBXN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18259

Product name: Recombinant human UBXN2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-170 aa of BC113645

Sequence: MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQ

相同性解析による交差性が予測される生物種
Full Name UBX domain protein 2B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC113645
Gene Symbol UBXN2B
Gene ID (NCBI) 137886
RRIDAB_3085772
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14CS0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

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