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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBXN2B Polyclonal antibody (25141-1-AP)

    WB analysis using 25141-1-AP (same clone as 25141-1-PBS)

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UBXN2B Polyclonal antibody (25141-1-AP)

    IHC staining of human ovary cancer using 25141-1-AP (same clone as 25141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using UBXN2B Polyclonal antibody (25141-1-AP)

    IF Staining of Neuro-2a using 25141-1-AP (same clone as 25141-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

UBXN2B Polyclonal antibody, PBS Only

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25141-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25141-1-PBS targets UBXN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18259

Product name: Recombinant human UBXN2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-170 aa of BC113645

Sequence: MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQ

相同性解析による交差性が予測される生物種
Full Name UBX domain protein 2B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC113645
Gene Symbol UBXN2B
Gene ID (NCBI) 137886
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14CS0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

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