Validation Data Gallery
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Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25141-1-AP targets UBXN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18259 Product name: Recombinant human UBXN2B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC113645 Sequence: MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQ 相同性解析による交差性が予測される生物種 |
| Full Name | UBX domain protein 2B |
| Calculated molecular weight | 331 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC113645 |
| Gene Symbol | UBXN2B |
| Gene ID (NCBI) | 137886 |
| RRID | AB_3085772 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14CS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UBXN2B antibody 25141-1-AP | Download protocol |
| IHC protocol for UBXN2B antibody 25141-1-AP | Download protocol |
| WB protocol for UBXN2B antibody 25141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |