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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using UBN1-Specific Polyclonal antibody (20363-1-AP)

    WB analysis of mouse liver using 20363-1-AP (same clone as 20363-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UBN1-Specific Polyclonal antibody (20363-1-AP)

    WB analysis of MCF-7 using 20363-1-AP (same clone as 20363-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UBN1-Specific Polyclonal antibody (20363-1-AP)

    IHC staining of human cervical cancer using 20363-1-AP (same clone as 20363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UBN1-Specific Polyclonal antibody (20363-1-AP)

    IHC staining of human cervical cancer using 20363-1-AP (same clone as 20363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20363-1-PBS in a different storage buffer formulation.

UBN1-Specific Polyclonal antibody, PBS Only

UBN1-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20363-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20363-1-PBS targets UBN1-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name ubinuclein 1
Calculated molecular weight 121 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberNM_016936
Gene Symbol UBN1
Gene ID (NCBI) 29855
RRIDAB_10694818
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPG3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.

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